Magnetic dextran nanoparticles ( -D and -D-spio)
are available with particle diameters of 130 nm, 250 nm and 500 nm for separation with a permanent magnet ( -D). Superparamagnetic dextran nanoparticles with particle diameters of 20 nm, 50 nm and 100 nm can not be separated with a conventional permanent magnet but in a high gradient magnetic field ( -D-spio).
Magnetic dextran nanoparticles ( -D):
are prepared via the core-shell method with a core of magnetite and a dextran shell,
consist of 75-80% (w/w) magnetite in a matrix of dextran (40.000 Da),
are designed with the surface functionalities OH (plain), NH2 and COOH for the covalent binding of proteins, antibodies or other molecules,
are available with covalently bound proteins (avidin, streptavidin, protein A, albumin) or other biomolecules (biotin, glutathion),
can be provided with covalently bound antibodies on request,
are offered with the nickel(II) chelator nitrilotriacetic acid (NTA) or ready to use with the corresponding nickel complex (Ni-NTA) for the binding of histidine labeled proteins,
are also suitable for the complexation of metal ions with covalently bound chelators on the particle surface (EDTA or DTPA),
are available with various hydrophilic surfaces (PEG 300, PEG-NH2 or PEG-COOH),
are available with negative surface potentials (e.g. COOH, SO3H).
Superparamagnetic dextran nanoparticles ( -D-spio):
are prepared by precipitation of iron oxide in the presence of dextran,
functionalized -D-spio consist of about 55-85% (w/w) iron oxide in a matrix of dextran (40.000 Da) in dependence on the diameter and surface modification,
differ in the size of the iron oxide domains for different diameters,
are preferably applied for detection purposes in magnetic resonance imaging or in magneto-immuno assays,
show good specific power absorption rates for hyperthermia applications for a particle diameter of 100 nm,
are designed with the surface functionalities OH (plain), NH2 and COOH for the covalent binding of proteins, antibodies or other molecules,
are available with covalently bound proteins (avidin, streptavidin, protein A, albumin) or other biomolecules (e.g. biotin),
are available with various hydrophilic surfaces (PEG 300, PEG-NH2 or PEG-COOH),
are available with negative surface potentials (e.g. COOH, SO3H) - can be provided with covalently bound antibodies on request.